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Y-27632 prevents TGFβ-induced E-cadherin and β-catenin delocalization from the cell membrane. Lens explants (n ≥ 5 per treatment) were fixed and costained for (A) E-cadherin and αSMA or (B) β-catenin and αSMA following TGFβ (4 ng/mL) treatment in the presence and absence of Y-27632 after 48 h. All explants were mounted in medium with DAPI to colocalize nuclei. Scale bar = 100 μm (A).
