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. 2016 Dec 2;11(12):e0167445. doi: 10.1371/journal.pone.0167445

Fig 3. Expression of Ado receptors in MDA-MB-231 cells.

Fig 3

(A). Percentage of MDA-MB-231 cell proliferation in the presence of Ado with or without dipyridamole (Dipy) an inhibitor of nucleoside transport. (B) RT-PCR of Ado receptor mRNAs in MDA-MB-231 cells. A representative gel is shown (upper panel). Levels of Ado receptor mRNAs were estimated from gene quantitative RT-PCR data and expressed as a percentage of β-actin mRNA expression (lower panel). Cells were incubated for 72 h in the absence (Control) or presence of Ado (50 μM), or the A2B receptor agonist Bay 60–6583 (1 μM). (C) Immunocytochemistry for the A2B receptor was performed to verify expression at the protein level in the absence (Ab -) or the presence of specific antibodies (Ab +).