Fig 9. Different SNPs (synonymous and non-synonymous) in the efpR gene reduce the efpR gene expression during growth in minimal medium.

Bacteria were grown in minimal medium supplemented with D-glucose (20 mM). Bacteria were harvested for total RNA extraction at OD_600nm ~ 0.5 and efpR mRNA were quantified using a qRT-PCR approach. Three biological repeats were conducted for each strain (two for the strain efpR ^P96Q). Bars indicate standard deviation (Welch t-test, comparison with the WT strain, * p-value <0.05, **p-value <0.01).