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. 2016 Dec 8;1(20):e90301. doi: 10.1172/jci.insight.90301

Figure 4. miR-323a-3p targets SMAD2 to suppress TGF-β signaling.

Figure 4

(A) miR-323a-3p binding sites in the SMAD2 3′ UTR. (B) Western blot for SMAD2 in cell lysates from 16HBE14o- cells transfected with miR-323a-3p mimics or control. A representative blot is presented from 3 individual experiments. (C) Luciferase activity of HEK cells transfected with a reporter plasmid containing a chimeric firefly luciferase with the SMAD2 3′ UTR. All data were normalized to Renilla luciferase activity. n = 3, *P < 0.001. (D and E) 16HBE14o- cells transfected with miR-323a-3p mimic or control were stimulated with TGF-β and processed for PCR for measuring levels of (D) CDH2 and (E) VIM. n = 4, *P < 0.01. Data are presented as box-and-whisker plots. The central horizontal bars indicate the medians, boxes indicate 25th to 75th percentiles, and whiskers indicate 1.5 times the interquartile range from the bottom and the top of the box. Statistical analysis in this figure was performed with the Student’s t test.