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. 2016 Oct 31;113(46):E7307–E7316. doi: 10.1073/pnas.1613530113

Fig. 5.

Fig. 5.

SP1’s function depends on its RING domain. (A) Sequence of SP1’s RING domain. The conserved active Cys and His residues are in red. The underlined residues were replaced in SP1m by the residues shown below in green. Amino acid numbers are indicated. (B) Four-week-old plants that had grown for 1 wk on LS medium and 3 wk on soil. (C and D) Sucrose-dependence assays. Seedlings were grown on MS medium supplemented with different concentrations of sucrose. Average root lengths of 10-d-old light-grown seedlings (C) and average hypocotyl length of 10-d-old dark-grown seedlings (D) are shown. Data are shown as mean ± SD; n = 3 (>30 seedlings for each biological replicate). ***P < 0.001; **P < 0.01; *P < 0.05 from pex14-2. (E) 2,4-DB response assays. Seedlings were grown on MS medium supplemented with 0.5% sucrose with or without 1.6 μM 2,4-DB. The average root length of 7-d-old light-grown seedlings is shown. Data are shown as mean ± SD; n = 3 (>30 seedlings for each biological replicate).