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In vivo characterization of CuMbn import. 65CuMbn uptake in (A) WT Ms. trichosporium OB3b and the mutant lacking MbnT (ΔmbnT) and (B) E. coli cells expressing MbnT or containing the empty pET-20b vector. Columns represent three averaged biological replicates, and error bars represent SD. Buffers used to wash the cells were used as controls. Uptake of 65CuMbn results in a decreased 63Cu:65Cu ratio.
