Table S1.

Summary of diffusivity of lipids, streptavidin, and rKin430-SBP-GFP for different SLB compositions

SLB composition (molar ratio)	Lipid diffusivity	Streptavidin	rKin430-SBP-GFP
	DLipid,* µm2/s (FRAP analysis)	DSA,† µm2/s (MSD analysis)	DKin,† µm2/s (MSD analysis)
DOPC:DSPE-PEG-2000-Biotin, 99:1	3.0 ± 0.3 (n = 16)	1.5 ± 0.1 (n = 136)	1.4 ± 0.2 (n = 196)
DOPC:Cholesterol:DSPE-PEG-2000-Biotin, 79:20:1	2.1 ± 0.4 (n = 16)	1.1 ± 0.2 (n = 171)	1.0 ± 0.2 (n = 48)
DOPC:Cholesterol:DSPE-PEG-2000-Biotin, 39:60:1	1.1 ± 0.2 (n = 16)	0.6 ± 0.1 (n = 160)	0.5 ± 0.1 (n = 58)

^*Values are mean ± SD (four independent experiments) obtained from the fit according to ref. 18.

^†Values are mean ± 95% confidence intervals obtained from the linear fit to the first eight points of the MSD as a function of time.