Skip to main content
. 2016 Oct 31;113(46):13069–13074. doi: 10.1073/pnas.1602393113

Fig. 3.

Fig. 3.

(A) Images of 5HT6-mCherry-cADDis–expressing cells with a cilium and in nonciliated cells (whole cell). The dotted lines represent the region of interest measured. The solid green line outlines the whole cell of the cell expressing the cilia-targeted 5HT6-mCherry-cADDis. (B) Dose response to the cell-permeable cAMP analog 8-Br-2′-O-Me-cAMP-AM. Cells expressing 5HT6-mCherry-cADDis were incubated with the cyclase inhibitor MDL-12330A to inhibit cAMP production, and then the sensor intensity was measured after addition of the cell-permeable cAMP analog (n = 3; 6 to 10 ROIs per point for each experiment). The mCherry/cADDis ratios were plotted versus [cAMP analog] to produce the dose response. (C) The ratio of mCherry to cADDis is higher in cilia compared with the whole cell, demonstrating that cilia have higher basal cAMP levels compared with the whole cell (*P < 0.05; n = 3 experiments). (D) Ciliary cAMP concentrations are five times higher than whole-cell levels, determined by interpolation of ratios on a standard curve (*P < 0.05; n = 3; 6 to 10 ROIs per experiment). Data are presented as mean ± SEM.