Fig. S4.
(A) Knockdown of Gαs prevents stimulation of β2AR with isoproterenol (1 μM) but not direct stimulation of adenylate cyclase with L-858051 (100 μM). (B) Time courses of live-cell fluorescence changes in cilia show cAMP increases in response to stimulation of 5HT6R with 2-methyl-5-hydroxytryptamine (100 nM), a Gαs-coupled receptor, and direct AC stimulation by L-858051 (100 μM). Both receptor and direct AC-stimulated cAMP production are inhibited by SHH. Gd3+ effectively inhibits the effect of SHH on cAMP production. (C) cAMP production with 3 μM L-858051 is inhibited by stimulation of the transfected Gαi/o-coupled NPY2 receptor with PYY (3-36) (100 μM) in the presence of boiled PTX (250 ng/mL) but not PTX (250 ng/mL). Data are presented as mean ± SEM.