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. 2016 Oct 31;113(46):13069–13074. doi: 10.1073/pnas.1602393113

Fig. S6.

Fig. S6.

PKD2 but not PKD2L1 was detected by RT-PCR in MEFs and IMCD3 cells. We used several other primer sets and still could not detect PKD2L1 in either cell type (not shown). Using a qPCR probe to PKD2L1, we did not detect significant mRNA expression in MEFs or IMCD3 cells (n = 2). We used three other qPCR probes to PKD2L1 and still failed to detect significant mRNA in IMCD3 cells. PKD2 was detected in both cell types. Calculating ΔCT as (CTPKD2 − CTGAPDH), in IMCD3 cells: ΔCT, 2.9; in MEFs: ΔCT, 4.2.