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. 2016 Nov 3;113(46):E7202–E7211. doi: 10.1073/pnas.1612820113

Fig. 3.

Fig. 3.

Expression in Et of engineered reporter BACs harboring the regulatory locus of Sp onecut. (A) Schematic of CB restriction in the Sp embryo. Red bars indicate repressor genes restricting CB genes in those domains. (B) Schematic showing the Sp onecut locus and the CRMs responsible for its geometric positioning. (C) Results of analysis of reporter expression of five Sp onecut BACs in Et. The BACs were previously used to analyze the cis-regulatory dynamics of onecut spatial control in Sp (65). (D) BAC reporter 1 harbors mutations to all known CRMs and shows markedly reduced reporter expression. (E) BAC reporter 2 harbors mutations to a zygotic activation CRM. (F) BAC reporter 4 harbors mutations to the AE and OE repression CRMs, is ectopically expressed in Et in OE and AE, and exhibits regular reporter expression in CB. (G) BAC reporter 3 harbors mutated postactivation enhancer CRMs and shows markedly reduced reporter expression. (H) BAC Reporter 5 harbors the unperturbed locus of Sp onecut and repeatedly exhibits reporter GFP in CB of Et.