Fig. 3.

Up-regulation of Rag1 and other genes associated with early B-cell development in Tg26 lymphoma. (A) The first 20 most up-regulated genes in Tg26 lymphoma lymph nodes and spleen (green) vs. Tg26 asymptomatic lymph nodes and spleen (healthy; blue) are shown. Nine of the 20 most highly up-regulated genes in lymphoma are important at the early B-cell stage, including Rag1 and Rag2. There were more than 1,000 significant genes with an FDR <1% and an absolute log twofold change >1; however, all 20 genes reported had an FDR <1e-10. The top 5% most differentially expressed genes are listed in Dataset S1, and the first 20 most down-regulated genes are presented in Fig. S5. (B) RAG1 protein is highly expressed in lymphoma tissues. Cell lysates from spleen and lymph node from indicated mice were probed by Western blot analysis for RAG1 and albumin.