Fig. S3.
Human PBMC subsets express receptors for p17. PBMCs were isolated from leukopaks from healthy donors (New York Blood Center) with Lymphoprep. After RBC lysis, cells were stained with p17-Alexa Fluor 430 and surface markers. Live cells were gated as follows: B cells, CD19+CD3−; T cells, CD19−CD3+; NK cells, CD56+CD3−; and monocytes, CD14+. The percent positive (Left) and median fluorescence intensity (MFI; Right) for p17 staining among each subset is plotted (n = 6). The majority of B cells and monocytes bound p17, as expected.