Fig. 3.

Leukocytes from the gene-edited fetus are resistant to M. haemolytica leukotoxin-induced cytolysis. Leukocytes from the gene-edited fetus and a wild-type calf were subjected to the MTT dye-reduction cytotoxicity assay with serial dilutions of M. haemolytica leukotoxin. Leukocytes from the wild-type calf (blue line) were lysed in a concentration-dependent manner, whereas the leukocytes from the gene-edited fetus (red line) were not lysed even by the undiluted leukotoxin. All data are expressed as mean ± SD (n = 3).