Fig. S5.

Roles of the actin and microtubule cytoskeletons in the spatial distribution of the mechanical properties of the cytoplasm. (A) Average values (normalized with respect to the control values) of the rigidity index, the bead step amplitude, the spring constant, and the viscosity in the presence of the indicated drug. Data are averages from n = 50, 43, 26, and 20 beads in the presence of nocodazole, Taxol, LatA, and CytoD, respectively. (B and C) Maps showing the distribution of the rigidity index and the bead step amplitude (B), and the spring constant and the viscosity deduced from the SLL analysis (C) in RPE1 control cells or in cells treated with the indicated drug destabilizing the microtubule or the actin cytoskeleton. (D) Autocorrelation length of the rigidity index maps. (E) Relative contributions of elastic storage and viscous loss to the global rigidity of the cytoplasm evaluated by the averaged ratios between G′ and G″ obtained by the PL analysis (Left) and by the ratio between the cross-correlation of the rigidity map with the spring constant map and the cross-correlation of the rigidity map with the viscosity map (Right) in each experimental condition.