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DIS-containing RNAs prepared with different deuterium labeling schemes can form heterodimers with kissing (A) or extended duplex (B) interfaces. The A268-H2 to G251-H1′ separation is too great for NOE detection in the kissing conformer but short enough to be readily detected in extended duplex conformer. (C) Portions of the 2D 1H–1H NOESY spectra obtained for uniformly protonated DIS237–281 oligo-RNA (AHCHGHUH) (Left) and an equimolar mixture of A2 and CrGrUr-labeled DIS237–281 (Right).
