Table S2.

Primers used for the PCR-based preparation of DNA templates for RNA transcription

Primer	Description	Sequence, 5′–3′*
5′-primer_80	Forward amplification primer for all 5′-L samples	GGGATGTGCTGCAAGGCGATTAAGTTGGG
R_TSL4nativeBlunt	Reverse amplification primer for 5′-L356 template	mUmACCGACGCTCTCGCACCCATCTCTC
R_TSL4-344	Reverse amplification primer for 5′-L344 and 5′-L344ΔPBS template	mCmGCACCCATCTCTCTCCTTCTAGCCTCC
R_TmSL4-344-lrAID	Reverse amplification primer for 5′-L344-UUA lrAID and 5′-L344-UUG lrAID template	mCmGCACTTATCTCTCTCCTTCTAGCCTCCGC
Top17	T7 promoter	TAATACGACTCACTATA
DIS_237-281	Template for transcription of DIS237–281 RNA	mGmGCCCCTCGCCTCTTGCCGTGCGCGCTTCAGCAAGCCGAGTCCTGCCTATAGTGAGTCGTATTA
U5-gaga-AUG-UUAlrAID	Template for transcription of U5:AUGUUA lrAID RNA	mGmGCGCACTTATCTTCTCAGATAAGCACACCTATAGTGAGTCGTATTA
U5-gaga-AUG-UUGlrAID	Template for transcription of U5:AUGUUG lrAID RNA	mGmGCGCACTTATCTTCTCAGACAAGCACACCTATAGTGAGTCGTATTA

^*m denotes the incorporation of 2′ O-methyl RNA bases.