Fig. 5.

WT161 enhances BTZ-induced ER stress and apoptotic cell death. (A) MM.1S cells were cultured with increasing concentrations of WT161 for 16 h in the presence or absence of BTZ. (B) Patient-derived MM cells were treated with DMSO (control; C), 2 µM tubacin (T), or 2 µM WT161 (161) in the absence or presence of BTZ for 24 h. (C) MM.1S cells were cultured with vehicle control, WT161 (3 μM), BTZ (3 nM), or BTZ+WT161 for 4 h and 8 h. Whole-cell lysates were subjected to immunoblotting with the indicated antibodies. (D) Patient-derived MM cells were treated with 5 nM BTZ ± 2 µM WT161 for 24 h. Whole-cell lysates were subjected to SDS/PAGE and immunoblotting with the indicated antibodies.