Fig. 7.

KDM3A and hypoxia-activated trophoblast-directed uterine spiral artery remodeling. (A) Schematic showing experimental plan for lentiviral transduction of blastocysts and in vivo transfer to pseudopregnant recipient animals. (B) Representative images of immunolocalization of KDM3A and pan cytokeratin (pKRT) on gd 13.5 placentation sites expressing control (Ctrl) shRNA or Kdm3a shRNA exposed to 10.5% O₂ tension from gd 6.5 to 13.5. (C) Immunolocalization of pKRT on gd 13.5 placentation sites expressing Ctrl shRNA or Kdm3a shRNA exposed to 10.5% O₂ tension from gd 6.5 to 13.5. (D) Quantification of the depth of cytokeratin-positive cell penetration into the uterine mesometrial vasculature (n = 6/group; *P < 0.05). (E) Localization of vimentin in placentation sites following Ctrl shRNA or Kdm3a shRNA transduction. (Scale bar, 1 mm.) Dashed black lines demarcate the location of the junctional zone (JZ) relative to the underlying labyrinth zone (LZ). (F) Ratio of cross-sectional areas of JZ vs. LZ from Ctrl shRNA and Kdm3a shRNA transduced placentation sites (n = 5/group; *P < 0.05). Data presented in D and F were analyzed with Student t test.