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. 2016 Oct 31;113(46):13150–13155. doi: 10.1073/pnas.1616336113

Fig. 1.

Fig. 1.

Prodigiosin specifically inhibits the Wnt/β-catenin signaling pathway. (A) Structure of prodigiosin and obatoclax. (B–F) The SuperTopFlash reporter gene was transfected into HEK293T cells together with empty vector or expression plasmids encoding Wnt1 (B); Wnt3 (C); Wnt1 and LRP6, Wnt3 and LRP6 (D); DVL2 (E); and β-catenin (F). (G) The SuperTopFlash reporter gene was transfected into HEK293T cells, after which the cells were treated with control or Wnt3A conditioned medium (Wnt3A-CM). (H) HEK293T cells were transfected with an AP1-Luc reporter and empty vector or a constitutively active Rasv12 expression plasmid. (I) HEK293T cells were transfected with a NFAT-Luc reporter and empty vector or an expression plasmid for NFATc. The transfected cells were incubated with vehicle or prodigiosin (25–500 nM) for 24 h. The luciferase values were normalized to β-gal activities.