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. 2016 Dec 2;2(12):e1600452. doi: 10.1126/sciadv.1600452

Fig. 3. Lifetime of receptor dimers and IFN signaling activity depend on MSK integrity.

Fig. 3

(A and B) Ternary complex lifetime in the native PM obtained from a colocomotion trajectory length histogram (390 individual tracked colocomotion events). An average lifetime of 15.2 ± 1.7 s (mean ± SD) was obtained by an exponential fit. (B) Complex lifetimes obtained from trajectory length histograms in the absence and in the presence of LatB. (C and D) IFNα2-stimulated translocation of STAT2 in the absence and in the presence of LatB. (C) Translocation of STAT2-TagRFP stably expressed in HeLa cells, visualized by confocal laser scanning microscopy. (D) Time-dependent STAT2 translocation monitored by the ratio of fluorescence intensity of STAT2-TagRFP in the nucleus to that in the cytoplasm. Data are presented as means ± SD from seven cells connected by lines to guide the eye. (E) Kinetics of STAT1 phosphorylation at different IFNα2 concentrations in the absence and in the presence of LatB. HeLa cells were stimulated with the indicated concentrations of IFNα2, and pSTAT1 was quantified at various time points by phosphoflow cytometry. Data are means ± SE from four independent replicates.