Skip to main content
. 2016 Dec 2;2(12):e1600452. doi: 10.1126/sciadv.1600452

Fig. 4. Modeling of diffusion and interaction in the context of a stochastic MSK meshwork.

Fig. 4

(A) Model of the MSK mesh with an average 70-nm length scale. Inset is a 2× magnification of the region highlighted by the rectangle. (B) Displacement probability density of the diffusing receptors (IFNAR1, circle; IFNAR2, triangle) in the PM of native cells (blue) and LatB-treated cells (red). Fitting the experimental data sets (dotted lines) with the MSK hop diffusion model yielded experimental hopping probability values summarized in Table 1. (C) Representative spatial stochastic simulation of an individual complex within MSK microcompartments, revealing transient dissociation and reassociation of receptor dimers (time lapse, 0.032 s; IFNAR1, cyan; binary IFNAR2/IFNα2 complex, magenta; ternary complex, blue). (D) Experimental lifetimes of ternary complexes measured in solid supported lipid bilayers (LB) and in the PM, compared to simulated ternary complex lifetimes based on receptor confinement by the MSK in native cells (blue) and in LatB-treated cells (red).