Figure 6.

Anti-MM activity of lycorine in MM xenograft mouse models. (A) Changes in tumor volume. Caliper measurement of the tumor diameters were performed very 4 days to estimate the tumor volume using the following formula (a×b²)/2, where 'a' and 'b' respectively are the longest and shortest perpendicular diameters of tumor. Data are presented as the mean±SD from five mice. (B) Representative tumor bearing mouse from each treatment group before dissection (top). The mice were euthanized at the treatment end point, tumors were removed and photographed (bottom). (C) During the treatment period, mouse body weight was measured every 4 days. Data are shown as mean±SD. (D) Histochemical examinations of mouse tissue section. Tissue sections from the tumor were fixed and stained with HE and TUNEL to examine the tumor cell morphology. The magnifications are 40×. (E) Western blotting analysis of the expression of HMGB1, Beclin-1 and LC3B in lysate extracted from tumor tissues. GAPDH was used as a loading control.