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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Bone Miner Res. 2016 Oct 31;31(12):2193–2203. doi: 10.1002/jbmr.2893

Figure 3. Decreased osteoblast and increased adipocyte differentiation in BMSC from Ddr2slie/slie mice.

Figure 3

BMSCs were isolated from 12 week-old wild type (WT) and homozygous Ddr2slie/slie female mice (DDR2) and grown in osteogenic (A–D) or adipogenic conditions (E–I). (A) Mineralization. Cells were stained with Alizarin red after 3 weeks in culture. (B–D) Osteoblast differentiation marker mRNAs. (B) Runx2 mRNA, (C) Bglap2 mRNA and (D) Ibsp mRNA. (E) Lipid droplet accumulation. Cells were stained with Oil red O after 9 days in culture. (F–I) Adipocyte differentiation marker mRNAs. (F) Pparg mRNA, (G) Cebpa mRNA, (H) Adipoq mRNA and (I) Fabp4 mRNA.