Table 1.
Generation of T Cells from Human Cell Sources
| Starting cell type | In vitro differentiation methods | Approx. fold expansion | Duration (weeks) | CD8+ T cells? | References |
|---|---|---|---|---|---|
| Cord blood HSC | OP9-DL co-culture | 10,000 | 7 | Yes | [17] |
| OP9-DL co-culture | 100,000 | 6 | Yes | [21] | |
| LSC-mDL1 co-culture | 50,000 | 10 | Yes | [22] | |
| Fibroblast-DL co-culture | 50,000,000 | 2 | No | [86] | |
| DL-coated ICC (feeder-free) | ~1.5 | 2 | Yes | [87] | |
| S17-DL co-culture | N.D. | 6 | Yes | [88] | |
| Conditioned medium and immobilized DL | N.D. | 3.5 | Yes | [27] | |
|
| |||||
| Bone marrow HSC | OP9-DL co-culture | 10,000 | 6 | Yes | [21] |
| LSC-mDL1 co-culture | 500 | 8 | Yes | [22] | |
| DL-coated microbeads | ~2 | 1 | N.D. | [26] | |
|
| |||||
| ESC | OP9-DL | N.D. | 4 | Yes | [38] |
|
| |||||
| iPSC | OP9-DL | ~25–150 | 4 | No | [38,43] |