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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cancer Res. 2016 Sep 7;76(23):6901–6910. doi: 10.1158/0008-5472.CAN-16-0517

Figure 5. Expression of CD166 on myeloma cells alters bone remodeling balance.

Figure 5

(A) BMSC were cocultured with H929 cells (as indicated) for 48h. H929 cells were removed and RNA was isolated from BMSC. Relative expression of RUNX2 RNA in BMSC was detected by quantitative PCR relative to GAPDH and the “WT BMSC alone” sample. (B) Calvariae were cocultured with H929 cells (as indicated) for 7 days. H929 cells were removed. Relative RANKL and OPG RNA expression in calvariae cells were examined by quantitative PCR and RANKL/OPG ratio was calculated. (C) Non-adherent BM derived macrophage from WT or CD166KO mice were cultured for 3 days to enrich for adherent BM macrophages (BMM). BMM were then cultured in α-MEM in the presence of recombinant mouse M-CSF (10 ng/ml) and RANKL (50ng/ml) for 7 days and mock H929 or CD166KD H929, followed by TRAP staining. TRAP-positive cells with 3 or more nuclei were scored under an inverted microscope. Data represent 3 separate experiments done in triplicates for each group and are expressed as mean± SEM, *p<0.05.