Table 1.
Antibodies Used in Immunohistochemistry1
| Antibody | Immunogen | Optimal [1°] | Source |
|---|---|---|---|
| Mouse anti-CALB | Bovine kidney calbindin-D-28K | 1:500 | C9848; Sigma-Aldrich, St. Louis, MO |
| Mouse anti-CART | Rat CART (54–102) conjugated to ovalbumin | 1:2000 | Dr. L. Thim, Novo Nordisk A/S, Bagsvaerd, Denmark |
| Rabbit anti-CCK | Sulfated CCK-8 (26–33) | 1:1000 | 20078; Incstar, Stillwater, MN |
| Rabbit anti-CR | recombinant human calretinin | 1:500 | 7699/3; Swant (Bellinzona, Switzerland); |
| Mouse anti-Leu-ENK | Leu5-enkephalin conjugated to bovine serum albumin | 1:1000 | MAS083; Sera-Lab; Crawley Down, UK; Cuello et al., 1984 |
| Sheep anti-GAD | Partially purified rat brain synaptosomes | 1:3000–4000 | 1440-4; National Institutes of Health; Oertel et al., 1981 |
| Rabbit anti-NPY | 36 amino acid sequence | 1:1,500 | RAS7172N; Peninsula Labs |
| Mouse anti-PARV | Purified frog muscle parvalbumin | 1:500–1:1000 | P3088; Sigma-Aldrich, St. Louis, MO; Heizmann et al., 1987 |
| Rat anti-SP | Carboxylic terminal fragment of human SP | 1:1000 | MAS035b; Sera-Lab, Crawley Down, Sussex, UK |
| Rabbit anti-TH | TH purified from bovine adrenal medulla | 1:1000–2000 | TE101; Eugene Tech, Allendale, NJ; Armstrong et al., 1981 |
| Rabbit anti-VIP | Carboxyl terminal 18–28 region of VIP | 1:1000 | 7916; Dr. J.H. Walsh, Univ. California Los Angeles |
1
For abbreviations, see text.