Histological evaluation of PanIN lesions in pancreata from 4-month (A, B) and 8-month old (C, D) KC and KC RictorΔ/Δ mice. (A) Representative images from H&E stained tissues, quadchrome stained tissues and Rictor RNAscope® stained tissues. The quadchrome stain marks collagen red, mucin blue, cytoplasm yellow-brown, and nucleic acids black. (B) Quantification of normal and neoplastic tissue as a percentage of total tissue area in 4-month old KC and KC RictorΔ/Δ pancreata. (C) Representative images from H&E stained tissues, quadchrome stained tissues and Rictor RNAscope® stained tissues. (D) Quantification of normal and neoplastic tissue as a percentage of total tissue area in 8-month old KC and KC RictorΔ/Δ pancreata. Student’s t test: *p<0.05, **p<0.01, ***p<0.001.