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. 2016 Nov 22;8(4):299–308. doi: 10.1007/s12551-016-0232-8

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Apparent weight-average molecular weight (M _w,app) of the CD2 and CD48 proteins and of the CD2–CD48 heterodimer as determined using sedimentation equilibrium × CD2, ● CD48 and O the CD2–CD48 heterodimer. Non-linear least-square fits to data for CD2 (dotted line) and CD48 (dashed line). Continuous line Predicted regression for a value of 2BM (from COVOL) of 10.4 ml/g. Dotted–dashed line Fit to CD2–CD48 heterodimer data. Using the COVOL value of 2BM = 10.4 ml/g, a value for the dissociation constant K_d ∼ (1.0 ± 0.3) × 10⁻⁴ M is obtained, a weak interaction. Figure is from Silkowski et al. (1997), with kind permission of the European Biophysics Journal (Springer Science + Business Media)