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View full-text article in PMC

. 2016 Dec 2;22:1375–1386.

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Copyright © 2016 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Islet-1 affects the invasion and migration of HUVECs in vitro. For assessment of the migration ability of human umbilical vein endothelial cell lines (HUVECs), the wound healing and Transwell assays were used. A, B: The siRNA-insulin gene enhancer protein ISL-1 (Islet-1) group cells exhibited a 36% decrease in closure compared with the negative control group (★p<0.05), suggesting a role for Islet-1 in the migration of HUVECs. C, D: A Matrigel invasion assay in the Transwell culture chambers was performed to determine the effect of Islet-1 on the in vitro invasion of HUVECs. The number of siRNA-Islet-1 group cells that passed through Matrigel was only 41% compared with the negative control group (★p<0.05). Together, these results support a critical role for Islet-1 in the motility of HUVECs.