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. 2016 Dec 2;22:1361–1374.

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Copyright © 2016 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Effect of PKR siRNA and PI3K inhibitor LY294002 on hypoxia-induced PKR, p-PI3K, p-Akt, and VEGF expression in RF/6A cells. A: DsRNA-activated protein kinase (PKR), phosphophosphatidylinositol 3-kinase (p-PI3K), phosphoprotein kinase B (p-Akt), and vascular endothelial growth factor (VEGF) expression was detected with western blotting in the RF/6A cells following PKR siRNA transfection after 48 h. C: The histogram shows the densitometric analysis of the average levels for PKR, p-PI3K, p-Akt, and VEGF to GAPDH. Cells transfected with scramble siRNA were used as the negative control. B: The western blot shows PKR, p-PI3K, p-Akt, and VEGF expression in RF/6A cells treated with the PI3K inhibitor LY294002 for 30 min. D: The histogram shows the densitometric analysis of the average levels of PKR, p-PI3K, p-Akt, and VEGF to GAPDH. Hypoxic cells were used as the negative control. *p<0.05, statistically significantly different compared to the respective controls. Values represent means ± SD.