Table 1.
General characteristics of male and female rats at 6, 12, and 18 months of age
| General characteristics | 6 months | 12 months | 18 months | Three-way ANOVA | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| F-INTACT | F-GONADX | M-INTACT | M-GONADX | F-INTACT | F-GONADX | M-INTACT | M-GONADX | F-INTACT | F-GONADX | M-INTACT | M-GONADX | ||
| BW (g) | 264.7 ± 18.1 | 287.8 ± 19.4 | 425.1 ± 11.8 | 398 ± 18.1 | 295.9 ± 20.9 | 350.6 ± 20.9 | 504.3 ± 18.3 | 427.1 ± 18.1 | 372.4 ± 18.1 | 422.1 ± 18.1 | 627.1 ± 24.3 | 533.4 ± 18.1 | 6 < 12 < 18*** F < M***; Sex*GONADX*** |
| Kidney left (g) | 0.8 ± 0.05 | 0.9 ± 0.05 | 1.4 ± 0.05 | 1.0 ± 0.05 | 1.1 ± 0.07 | 0.9 ± 0.07 | 1.5 ± 0.05 | 1.1 ± 0.05 | 1.0 ± 0.05 | 1.0 ± 0.05 | 1.5 ± 0.05 | 1.1 ± 0.05 | 6 < 12,18***; F < M***; Sham > GONADX***; Gender*GONADX*** |
| Heart (g) | 0.9 ± 0.06 | 0.9 ± 0.06 | 1.5 ± 0.06 | 1.1 ± 0.06 | 1.2 ± 0.06 | 0.9 ± 0.07 | 1.2 ± 0.06 | 1.0 ± 0.06 | 1.1 ± 0.06 | 1.0 ± 0.06 | 1.5 ± 0.06 | 1.2 ± 0.06 | 6 > 12 < 18***; F < M***; Sham > GONADX***; Gender*GONADX*** |
| Urine volume (ml/24 h) | 11.0 ± 1 | 9.9 ± 1 | 11.1 ± 1 | 9.7 ± 1 | 13.4 ± 1 | 10.1 ± 2 | 10.1 ± 1 | 10. ± 1 | 10.9 ± 1 | 7.9 ± 1 | 11.3 ± 1 | 15.2 ± 1 | Sex*GONADX*, Sex*Age* |
| Water intake (ml/24 h) | 27.5 ± 2 | 18.3 ± 2 | 22.8 ± 2 | 20.6 ± 2 | 26.2 ± 3 | 20.9 ± 3 | 23.6 ± 2 | 21.1 ± 2 | 24.9 ± 2 | 18.9 ± 2 | 22.1 ± 2 | 24.4 ± 2 | Sham > GONADX***, Sex*GONADX* |
Animals underwent sham (INTACT) or ovariectomy or castration (GONADX) surgery at 10 weeks of postnatal age. The three-way ANOVA assessed the main factor effects of age, sex steroid and surgery, with Tukey HSD post hoc analysis where applicable. Data are presented as mean ± SEM for n = 8 animal per group
FI female INTACT, FG female GONADX, MI male INTACT, MG male GONADX,
*P < 0.05; ***P < 0.001