Fig. 2.

Effects of 70% ethanolic extracts of yuja peel (YE) on AMPK signalling and expression of proteins associated with cholesterol metabolism. HepG2cells were treated for 1 h with YE in the presence or absence of oleic acid, and then western blot analysis was performed with phospho-specific ACC, AMPK, normal AMPK, and beta-actin antibodies (a). HepG2cells were treated for 24 h with YE in the presence or absence of oleic acid, followed by western blot analysis with 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), peroxisome proliferator-activated receptor (PPAR)-α, carnitine palmitoyltransferase (CPT)-1, and beta-actin antibodies (b)