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. 2016 Dec 3;15:77. doi: 10.1186/s12943-016-0564-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — EMT changes depend on the LCN2/NF-κB/Snail pathway. a, b Changes of EMT markers measured by western blots and real-time PCR after enhancing NF-ĸB activity with LMB (40nM for 6 h), and quantification of relative mRNA expression of EMT markers in SW620-LCN2 cells (a) and suppressing NF-κB activity with specific siRNA (50nM for 48 h), and quantification of relative mRNA expression of EMT markers in SW480-sh-LCN2 cells (b), The grey value ratios of the corresponding proteins/GAPDH were shown. Values shown in real-time PCR assay are the mean ± SD from at least three independent experiments. (N, nuclear; C, cytoplasm; P, phosphorylated; NC, negative control). * P < .05, ** P < .01, *** P < .001