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. 2016 Dec 3;15:77. doi: 10.1186/s12943-016-0564-9

Fig. 7.

Fig. 7

EMT changes depend on the LCN2/NF-κB/Snail pathway. a Changes of EMT markers measured by western blots and real-time PCR after restoring snail expression, and quantification of relative mRNA expression of EMT markers in SW620-LCN2 cells. Snail protein (55KD) is a fusion protein with green fluorescence protein (GFP). The grey value ratios of the corresponding proteins/GAPDH were shown. b Migration assay and quantification of migration after overexpression of snail in SW620-LCN2 cells (OD values were measured by a microplate reader at 570 nm). Values shown in real-time PCR assay are the mean ± SD from at least three independent experiments. (N, nuclear; C, cytoplasm; P, phosphorylated; over, overexpression). * P < .05, ** P < .01, *** P < .001. Scale bars, 100 μm