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. 2016 Dec 1;4:e2661. doi: 10.7717/peerj.2661

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©2016 Neches et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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A total of 10 µl of each AYE tube (positive control, PLA plastic and negative control) was struck out on Charcoal Yeast Extract solid media and incubated at 37 °C to grow for 24 h. Growth revealed that the PLA test part (A) appeared to contain a different bacterial species than the positive control tube (B). Media from the negative control part was also plated (C). Using a light microscope, both bacterial growths appear coccoid, with the yellow colonies forming clumps more often. Experiment was repeated with parts from UC Davis and Michigan State, plus controls. Contamination was not observed.