Table 1.
strains and plasmids
| Strain or plasmid | Genotype or characteristics | Source or ref. |
|---|---|---|
| MG1655 | Wild type E. coli | (43) |
| LC106 | Δ(katG17::Tn10)1 Δ(ahpC-ahpF') kan::'ahpF Δ(katE12::Tn10)1 | (10) |
| JH400 | Δ(dgs-fumCA-manA)1 Δ(mel-fumB) zdg232::Tn10 metB1 spoT1 relA | John Guest |
| OD570 | ΔfumC::cm | (19) |
| OD571 | ΔfumCA::cm | (19) |
| SJ19 | ΔfumC::cm in LC106 | This study |
| SJ20 | ΔfumAC::cm in LC106 | This study |
| SJ34 | ΔfumB::cm in BW25113 | This study |
| SJ37 | As LC106 plus ΔfumC ΔfumB::cm | This study |
| SJ38 | As LC106 plus ΔfumAC ΔfumB::cm | This study |
| BW25113 | lacIq rrnBT14 ΔacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | (12) |
| pWKS30 | Plac polylinker Amr | (18) |
| pLEUCD2 | pWKS30 containing leuCD insert | This study |
| pCKR101 | Plac–lacIq Ptac polylinker Amr | Jeff Gardner |
| pLEUCD3 | pCKR101 containing leuCD insert | This study |
| pFUMA | pCKR101 containing fumA insert | This study |
| pCP20 | temperature-sensitive FLP expression plasmid | (44) |
| pKD3 | FRT-flanked cat | (12) |
| pKD46 | λ Red recombinase expression plasmid | (12) |