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. Author manuscript; available in PMC: 2017 Feb 28.
Published in final edited form as: Nature. 2016 Aug 31;537(7621):544–547. doi: 10.1038/nature19353

Extended Data Figure 5. EMT signature in Fh1-reconstituted cells.

Extended Data Figure 5

a, Fh1 protein levels measured by western blot. Calnexin was used as loading control. b, Intracellular fumarate levels the measured by LCMS. Data are presented as average ± S.D.. c, Representative bright field images of cells of the indicated genotype. Scale Bar = 400 µm. d, e, mRNA expression measured by qPCR (d) and protein levels measured by western blot (e) of the indicated EMT markers. f, Average speed of cells calculated after tracking cells for 3 hours as in Fig. 1g. Results were generated from 3 independent cultures. g, mRNA expression of EMT-related transcription factors. β-actin was used as endogenous control. EV = empty vector. h, Expression levels of the indicated miRNAs measured by qPCR and normalised to Snord95 and Snord61 as endogenous control. All qPCR results were obtained from 3 independent cultures and presented as RQ with max values. *P ≤0.05, **P ≤0.01, ***P ≤0.001, ****P≤0.0001. Western blot sources are presented in Supplementary Figure 1. Raw data are presented in SI Table 2.