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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):1950–1961. doi: 10.1016/j.celrep.2016.10.062

Figure 2. Overexpression of KIF5B-KLC2-SKIP, KIF1Bβ or KIF1A causes accumulation of lysosomes at the cell periphery.

Figure 2

(A,B) HeLa cells expressing GFP-KIF5B, alone or in combinations with HA-KLC2 and SKIP-myc, were analyzed by immunofluorescence microscopy (A), and mean fractional distance ± SEM of lysosomes from the cell center was quantified from 30 cells in three independent experiments (B). (C-F) HeLa cells expressing FLAG-KIF1Bβ, GFP-KIF1A, or GFP-KIF1A deletion mutants were analyzed by immunofluorescence microscopy (C,E) and mean fractional distance ± SEM of lysosomes from the cell center was quantified as above (D,F). In A, C and E, lysosomes were stained with antibody to endogenous LAMTOR4, and nuclei were stained with DAPI (blue). Scale bars, 10 μm. Arrows point to cell protrusions where proteins co-localize.