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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):1950–1961. doi: 10.1016/j.celrep.2016.10.062

Figure 3. Peripheral accumulation of lysosomes driven by both KIF5B-KLC2-SKIP and KIF1A is dependent on Arl8a/b and BORC.

Figure 3

(A-D) Immunofluorescence microscopy of lysosomes stained with antibody to endogenous LAMTOR4 in WT, Arl8b-KO, Arl8b-KO–Arl8a-KD, myrlysin-KO and diaskedin-KO HeLa cells expressing GFP-KIF5B+HA-KLC2+SKIP-myc or GFP-KIF1A. Nuclei were stained with DAPI (blue). Scale bars: 10 μm. Arrows point to cell protrusions where proteins co-localize. (E,F) Quantification of mean fractional distance ± SEM of lysosomes from the cell center from 30 cells in three independent experiments such as those in A-D.