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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: J Immunol. 2016 Nov 11;197(12):4651–4662. doi: 10.4049/jimmunol.1600206

Figure 1. Cre-lox deletion of KLF2 selectively depletes Klf2 expression from CD11c expressing cells, and enhances co-stimulatory molecule expression in DCs following activation.

Figure 1

A, BMDCs were generated from Klf2fl/fl (Cntrl, white bars) or Itgaxcre-Klf2fl/fl (KO, black bars) bone marrow, matured with LPS alone or LPS plus simvastatin/rapamycin, and Klf2 mRNA were measured by qRT-PCR. Klf2 mRNA was measured in purified splenic naïve CD4+ T cells (B), peritoneal macrophages (C), and untreated (NT) or simvastatin/rapamycin-treated (S/R) peritoneal macrophages (D). E, CD40 and CD86 expression on LPS-matured CD11c+MHC-II+ BMDCs was measured by flow cytometry. Histograms show representative staining from 4 independent experiments. Bar graphs quantify CD40 and CD86 mean fluorescence intensity (MFI) normalized to control. A, n=10; B-D, n= 3; E-F, n=6. *, p<0.05; **, p<0.01; ***, p<0.001