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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: J Immunol. 2016 Nov 11;197(12):4651–4662. doi: 10.4049/jimmunol.1600206

Figure 2. Loss of KLF2 in DCs induces leukopenia and promotes a more pro-atherogenic inflammatory monocyte phenotype under hypercholesterolemic conditions.

Figure 2

Itgaxcre-Klf2fl/fl (KO) or Klf2fl/fl (Cntrl) bone marrow was transplanted into Ldlr−/− recipients and fed a high-fat diet for 10 weeks. A–B, qRT-PCR for Klf2 mRNA was performed on BMDC and macrophages (BM-Mɸ) generated from Cntrl and KO bone marrow recipients following sacrifice. C–D, Flow cytometry was performed on blood to determine circulating levels of mature DCs (C), and DC precursors (D). E–G, Circulating monocytes were stained for Ly-6C and CD11c expression. E and F show percentage of Ly-6Chi and CD11c+ monocytes respectively. G, shows a representative flow cytometry plot for Ly-6C and CD11c staining. H–J, splenocytes were stained for macrophage markers CD11b and F4/80. H, shows macrophages as percent of total splenocytes and I shows splenic macrophage numbers calculated from total splenocyte counts. J, shows a representative splenic F4/80, CD11b flow cytometry plot. A, n=11; B, n=6; C–J n=10–12. *, p<0.05; **, p<0.01; ****, p<0.0001