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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: J Immunol. 2016 Nov 11;197(12):4651–4662. doi: 10.4049/jimmunol.1600206

Figure 3. Loss of CD11C-KLF2 aggravates atherosclerosis.

Figure 3

Klf2fl/flCd11c-Cre−/− (Cntrl) or Klf2fl/flCd11c-Cre+/− (KO) bone marrow was transplanted into 12 male and 12 female Ldlr−/− recipients and fed a high-fat diet for 10 weeks. A, Shows animal weight at sacrifice. B–C, Blood serum was taken at sacrifice and lipid levels measured to determine total cholesterol and triglyceride levels. D–F, Frozen sections from aortic sinus were obtained and atherosclerotic lesions from the 3-valve region were stained with Oil Red O for neutron lipid (D), or by immunohistochemistry with antibodies specific for macrophage marker MAC-3 (E), anti-neutrophil antibody (NIMP-R14) (F). Nuclei were counterstained with hematoxylin. Isotype controls for MAC-3 and NIMP-R14 staining was negative (not shown). Bar graphs indicate lesion area as percent of lumen for male or female mice. Scale bars = 500 μm. A–C, n=12; D, n=20; E, n=12; F, n=6. *, p<0.05