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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Cancer Res. 2016 Sep 21;14(12):1288–1299. doi: 10.1158/1541-7786.MCR-16-0103

Figure 1. rVAR2 impedes attachment and detachment mechanics in tumor cells.

Figure 1

A, U2OS cells were preincubated with rVAR2 and tested for their capacity to adhere to Fibronectin (FN), Collagen I (C-I), Collagen IV (C-IV), and Plastic (PL). Adherent cells were stained with Methylene Blue and quantified by reading absorbance at 650nm. B, MG63 cells were preincubated with rVAR2 and tested for their capacity to adhere to Fibronectin (FN), Collagen I (C-I), Collagen IV (C-IV), and Plastic (PL). Adherent cells were stained with Methylene Blue and quantified by reading absorbance at 650nm. C, RH30 cells were preincubated with rVAR2 and tested for their capacity to adhere to Fibronectin (FN), Collagen I (C-I), Collagen IV (C-IV), and Plastic (PL). Adherent cells were stained with Methylene Blue and quantified by reading absorbance at 650nm. D, MDA-MB-231 cells were preincubated with rVAR2 and tested for their capacity to adhere to Fibronectin (FN), Collagen I (C-I), Collagen IV (C-IV), and Plastic (PL). Adherent cells were stained with Methylene Blue and quantified by reading absorbance at 650nm. E, U2OS cells were preincubated with rVAR2 or rContrl and allowed to adhere to plastic. The phosphorylation of Src and Erk1/2 kinases in response to the adhesion event was monitored by Western.