RAMP2-AS1 interacts with DHC10. A, Silver-stained SDS-PAGE gel analysis of proteins in nuclear extract of U87 cells that are bound to biotinylated RAMP2-AS1 or its antisense. The highlighted regions were analyzed by mass spectrometry, identifying DHC10 as a protein unique to RAMP2-AS1 (left). Immunoblotting analysis of proteins in nuclear extract of U87 cells that are bound to biotinylated RAMP2-AS1 or its antisense using an anti-DHC10 antibody (right). B, NOTCH3 expression in U87 cells receiving LV, LV-RAMP2-AS1, scramble siRNA(scrb si), or DHC10siRNA in combination as indicated (Error bars represent SD, *p<0.05).