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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2016 Oct 7;15(12):2863–2874. doi: 10.1158/1535-7163.MCT-16-0515

Figure 7. Effect of treatment with fisetin, cabazitaxel and their combination on on colony formation, apoptosis and P-gp in drug resistant NCI/ADR-RES cells.

Figure 7

A. Representative photographs showing the effect of fisetin treatment on NCI/ADR-RES cell growth investigated by monolayer colony formation assay. B. The induction of apoptosis in NCI/ADR-RES cells was assessed by flow cytometry using a FITC conjugated Annexin V (x-axis) marker coupled with propidium iodide (PI, y-axis) exclusion, below, quantitation of apoptosis ***, p ≤ 0.001. C. Representative blots showing the effect of fisetin and cabazitaxel treatment on P-gp activity. Data are shown as representative of three independent experiments. GAPDH served as a loading control. Bands were measured using densitometry and values first normalized to respective GAPDH bands and then reported below each gel as relative to control. Data are shown as representative of three independent experiments for each assay.