Figure 4. Knockdown of SKP2 negates the effects of AMPKα2 mediated control of p27 and cell proliferation.
A-B. HTB2 shGFP, HTB2 shAMPKα2, HT1376 shGFP and HT1376 shAMPKα2 cells were transfected with siRNA(100nM) to luciferase or SKP2 and assessed for SKP2, p27 and β-actin levels by immunoblot 72 hours post transfection. C-D. Day 3 cell counts compared to day 0 cell counts of transduced cells transfected with siLuc or siSKP2. Statistical significance indicated as; *p<0.05