Figure 1. Schematic representation of centromeres in fission yeast and the development of repeat-specific reporter.

Central core regions (cnt) are flanked by innermost repeats (imr) and outermost repeats (otr). Each of otr repeats consists of dg and dh repeat units as indicated. CENP-A^cnp1 is enriched in cnt and imr regions, while otr repeats are organized into peri-centromeric heterochromatin. The ura4⁺ reporter flanked with part of dg sequence was randomly inserted into otr repeats by homologues recombination. The location of ura4⁺ insertion is mapped by estimating the distance between two unique restriction sites in ura4⁺ and a heterochromatin boundary region, respectively (indicated by two scissors).