Table 3.

Reproducibility of the biofilm models utilised, as analysed by different analytical, imaging, and microbiological assays.

Model	Strain	Analysis method	n	Average	Std Dev
Simple biofilm model	Antibiotic-resistant P. aeruginosa NCTC 8506	Elemental analysis (values minus blank filter disc elemental analysis)	24	26.8 µM Mg2+	3.2 µM
				36.2 µM K+	7.1 µM
				171.9 µM Ca2+	27.0 µM
	Antibiotic-resistant P. aeruginosa NCTC 13437	Live/dead image analysis by pixilation	19	25.6 µm thick	8.2 µm
Simulated wound polymicrobial biofilm model	S. aureus, K. pneumoniae, 24-hour control	Confocal imaging of maximum depth	15	9.74 µm thick	2.37 µm
		Total viable counts	3	9.4 × 108 cfu S. aureus	2.0 × 108 cfu
				7.7 × 109 cfu K. pneumoniae	3.2 × 109 cfu