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. 2016 Sep 18;9(10):e35591. doi: 10.5812/jjm.35591

Table 1. List of Primers, Size of Amplicons, and PCR Conditions Used in this Studya.

Primers Sequences Amplicon Size PCR Conditions
cdt A gene cytA1: 5’-TGCAAAAAATTATAGTTTTTATTTTATGTTG-3’; cytA2: 5’-TCGTACCTCTCCTTGGCG-3’ 807 pb 94°C 1 minute; 62°C 1 minute;72°C 1 min; (30 cycles)
cdt B gene cytB1: 5’-ATGAAAAAAATTATATGTTTATTTTTATC-3’; cytB2: 5’-TTTTCTAAAATTTACTGGAAAATG -3’ 781pb 94°C 1 minute; 55°C 1 minute; 72°C 1 minute; (30 cycles)
cdt C gene cyt C1: 5’-ATGAAAAAAATTATTACTTTGTTTTTTATG-3’; cytC2: 5’-TTCTAAAGGGGTAGCAGCTG-3’ 570 pb 94°C 1 minute; 57°C 1 minute; 72°C 1 minute; (30 cycles)

aAll primers were designed for this study.