Table 1. List of Primers, Size of Amplicons, and PCR Conditions Used in this Studya.
Primers | Sequences | Amplicon Size | PCR Conditions |
---|---|---|---|
cdt A gene | cytA1: 5’-TGCAAAAAATTATAGTTTTTATTTTATGTTG-3’; cytA2: 5’-TCGTACCTCTCCTTGGCG-3’ | 807 pb | 94°C 1 minute; 62°C 1 minute;72°C 1 min; (30 cycles) |
cdt B gene | cytB1: 5’-ATGAAAAAAATTATATGTTTATTTTTATC-3’; cytB2: 5’-TTTTCTAAAATTTACTGGAAAATG -3’ | 781pb | 94°C 1 minute; 55°C 1 minute; 72°C 1 minute; (30 cycles) |
cdt C gene | cyt C1: 5’-ATGAAAAAAATTATTACTTTGTTTTTTATG-3’; cytC2: 5’-TTCTAAAGGGGTAGCAGCTG-3’ | 570 pb | 94°C 1 minute; 57°C 1 minute; 72°C 1 minute; (30 cycles) |
aAll primers were designed for this study.